Borth, W. B., D. E. Gardner, and T. L. German. 1988. Closterovirus associated with Dodonaea yellows disease. Phytopathology 78:1586.
ABSTRACT
A recently described lethal yellowing disease affecting Dodonaea eriocarpa in Hawaii superficially resembles diseases caused by mycoplasma-like organisms although the etiology of this disease remains unknown. Virus-like particles have been partially purified from symptomatic stems and leaf tissues of diseased plants. The purification protocol involves homogenization of tissues in Tris buffer + NaCl, precipitation by polyethylene glycol and isopycnic centrifugation in CsSO4/sucrose gradients. Using virions purified by this procedure, the molecular weight of the virion coat protein determined by SDS-PAGE electrophoresis is 23,000 daltons. Double-stranded RNA isolated from symptomatic tissues has a molecular weight of 3 x 106 daltons determined by PAGE. These characteristics suggest a possible closterovirus etiology for the disease.
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